Flow cytometry is a unique technique used by scientists to analyze and quantify different components of cells within a sample. This technique is very useful, because it enables researchers to determine the number of cells and the size and shape of the cells from a sample. One more flow cytometry consideration is determining if the cells are alive or not. To accomplish this, the scientists run the cells through a laser beam. The cells reflect red light absorbed by the laser. The reflected light is measured and recorded. With this information, researchers can extract a great deal of knowledge about the cell populations under study and their nature. The imaging flow cytometry is a popular method used in biological and medical applications. It helps scientists study how cells work and what they need to survive. This procedure is particularly significant in numerous domains including immunology (the study of the immune system), hematology (the study of blood), and recent research in malignant neoplasm. Here are some things that scientists need to consider when doing flow cytometry experiments:
Properly preparing the sample is step one. This means selecting the correct liquid, called a buffer, and the right color dyes to stain the cells. Step 2 is data acquisition. This is the part where the sample runs through the flow cell flow cytometry, which is where the actual measurements are taken. Last but not least, step four is data analysis. During this stage researchers find useful information from the data they collected which facilitates them in their research. However, there are times that flow cytometry experiments can get into trouble, and it is critical that scientists learn how to troubleshoot these situations. Common problems that may happen during flow cytometry are cell clumps, broken instruments, and mixed-up signals. Cell clumps occur when cells come together, which can interfere with accurate measurement. To reduce clumping, scientists can separate the cells before testing by using a device known as a cell strainer.
In case the machine has some issues, for example, it fails to work properly, then it is recommended to contact a specialist who will assist in its repair. One more problem that would crop up is signal interference. This means that extraneous signals can clear the results. To mitigate this, one should set up the multicolor flow cytometry carefully with minimal background noise during a given experiment, especially with auditory data.
Examples of two important dyes used in single cell flow cytometry (HC) are Hoechst 33342 and Propidium iodide (PI). Hoechst 33342 is a bright dye scientists use to mark live cells. It can also help ascertain whether the cells are healthy and alive. In contrast, PI is a marker for ineffective cells, as it marks damaged DNA. When PI binds to the DNA, it causes the dead cells to glow red, allowing scientists to easily identify them.
Hoechst 33342 is also helpful for scientists, as it can be used when they need to verify whether or not cells are alive during flow cytometry tests. In this way, researchers stain the cells with microfluidic flow cytometry and it is combined with other bright dyes to measure the cell viability, that is, I mean if the cells are alive or dead. This technique can also inform scientists about how the cells were growing and whether they were undergoing a process called apoptosis, when a cell dies in a safe and natural way.
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With more than 50 years of R&D and manufacturing experience, Jinko Optics has accumulated rich technical and practical knowledge in the field of spectral accessories. For a long time, focusing on the research and development of core products such as cuvettes, flow cells, optical components, and vapor cells has not only improved the company's technical position in the industry, but also enabled the company to quickly respond to various complex application requirements. The accumulation over the years has helped the company to continue to innovate and always be at the forefront of the industry.
Jinko Optics is committed to providing customers with high-quality products with high cost performance. By optimizing production processes and management processes and reducing production costs, the company can provide more favorable prices while ensuring the excellent performance of products in quality and function. In addition to the excellent quality of the products themselves, the company also pays special attention to after-sales service, providing timely technical support and professional solutions to ensure that problems encountered by customers during use are quickly resolved. This customer-oriented service concept enables Jinko Optics to stand out in the fiercely competitive market and win the trust and praise of a wide range of customers.
As the drafting unit of the national standard for cuvettes, Jinko Optics has very high standards for product quality. Every cuvette and optical component produced by the company follows the ISO9001:2016 standard, strictly controls every link in the production process, from the selection of raw materials to the factory inspection of finished products, to ensure that every product meets high quality requirements. In addition, it has 6 invention patents and 16 utility model patents, reflecting the company's continued investment in technological innovation and process optimization, so that the products not only have excellent performance, but also have unique market competitiveness.
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